치주인대세포에서 Aggregatibacter actinomycetemcomitans의 IL-8 및 활성산소종 유도능
Induction of IL-8 and reactive oxygen species in periodontal Ligament cells by Aggregatibacter actinomycetemcomitans
이양신, 유윤정, 차정헌, 박홍규, 김성환,
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이양신 ( Lee Yang-Sin ) - 연세대학교 치과대학 구강생물학교실
유윤정 ( Yu Yoon-Jeong ) - 연세대학교 치과대학 구강생물학교실
차정헌 ( Cha Jeong-Heon ) - 연세대학교 치과대학 구강생물학교실
박홍규 ( Park Hong-Gyu ) - 연세대학교 치과대학 구강생물학교실
김성환 ( Kim Sung-Whan ) - 연세대학교 치과대학 구강과학연구소
KMID : 0363020090390030331
Abstract
Purpose: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells.
Methods: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS.
Results: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans.
Conclusions: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
키워드
Aggregatibacter actinomycetemcomitans;interleukin-8;periodontal Ligament;reactive oxygen species
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